Description
HieffTM anti-Taq DNA Polymerase Antibody is a Taq DNA Polymerase monoclonal antibody for hot start PCR. It can inhibit the activity of DNA polymerase after binding to Taq DNA Polymerase, which can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification caused by primer dimer at low temperature. HieffTM anti-Taq DNA Polymerase Antibody is denatured in the initial DNA denaturation step of PCR reaction, and the activity of DNA polymerase is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibody.
Features
- Exceptional detection sensitivity—The hot-start formulation ensures effective detection of low-abundance genes, offering higher sensitivity.
- Rapid enzyme activity release—Over 95% of enzyme activity can be released by heating at 95°C for 20 seconds.
- No Nuclease Contamination: Free from contamination of exonucleases, endonucleases, and RNases.
Applications
The concentration of this product is 5 mg/mL. 1 μL antibody could block the activity of 20-50 U Taq DNA polymerase. It is recommended to mix the antibody and the Taq DNA polymerase well and incubate at room temperature for 20 min prior to use. The blocking efficiency is more than 90%.
Specifications
|
Classification |
Monoclonal |
|
Type |
Antibody |
|
Antigen |
Taq DNA Polymerase |
|
Form |
Liquid |
Components
|
31301ES60 （100 µg） |
31301ES80 （1 mg） |
31301ES90 （5 mg） |
|
|
HieffTM anti-Taq DNA Polymerase Antibody |
20 μL |
200 μL |
1 mL |
|
Name |
31301ES92 （10 mg） |
31301ES93 （100 mg） |
31301ES94 （1000 mg） |
|
HieffTM anti-Taq DNA Polymerase Antibody |
2×1 mL |
20 mL |
200 mL |
Storage
The product should be stored at -25°C~-15℃ for 2 years.
Documents:
Safety Data Sheet
EN-31301-MSDS-Ver.EN20250207.pdf
Manuals
EN_31301_Manual_Ver.EN20250207.pdf
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.