Description
Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody is a mixture of two monoclonal antibodies who can inhibit the activity of 5 '- 3' polymerase and 5 '- 3' exonuclease of Taq DNA Polymerase, respectively. The antibody can effectively inhibit the nonspecific annealing of primers and the nonspecific amplification. In addition, the product can effectively prevent probe degradation. Hieff™ Double-Block anti-Taq DNA Polymerase Antibody is denatured in the initial DNA denaturation step of PCR reaction, through which the activity of DNA polymerase is restored to achieve the effect of hot start PCR. It can be used under the condition of routine PCR reaction without special inactivation of antibodies.
Applications
Hot-start PCR
Compatible with Taq mutants
Components
| Component | Name | 31303ES60 | 31303ES80 | 31303ES90 | 31303ES92 | 31303ES93 |
| 31303 | Hieff™ Double-Block Anti-Taq DNA Polymerase Antibody (5 mg/mL) |
100 µg 400 U (20 μL) |
1 mg 4KU (200 μL) |
5 mg 20KU (1 mL) |
10 mg 40KU (2 mL) |
100 mg 400KU (20 mL) |
Specifications
| Classification | Monoclonal |
| Type | Antibody |
| Antigen | Taq DNA Polymerase |
| Form | Liquid |
| Concentration | 5 mg/mL, 20U/μL |
| Unit | One unit is defined as the amount of antibody required to inhibit greater than 90% one unit of Taq DNA polymerase at room temperature for 1 hour. |
A total of 1 μL antibody could block the activity of 20 U of Taq DNA polymerase. It is recommended to mix the antibody and the Taq DNA polymerase at room temperature for 1 hour (incubate at room temperature for 2 hours when volume is greater than 200 mL, and customer should adjust the process when applied to larger volume), and then store at -20℃ overnight before use.
Note: The specific activity of different Taq DNA Polymerase is variant, the blocking ratio needs to be adjusted appropriately to achieve that the blocking efficiency is better than 95%.
Shipping and Storage
The product is shipped with ice packs and can be stored at -25°C~-15℃ for 2 years.
Notes: Please wear the necessary PPE, such lab coat and gloves, to ensure your health and safety!
Figure
Fig 1. Detection of blocking efficiency of double-block antibody for exonuclease activity; Yellow: unclosed Taq DNA polymerase group; Purple: Taq DNA polymerase group blocked by double-block antibody