Description
Protein A/G MagBeads use "nano-surface biotechnology" (S-TEC) to target Protein A/G onto the surface of superparamagnetic polymer microspheres with high density. It has higher antibody binding ability and very low non-specific adsorption rate of protein. One-step purification can isolate the antibody with purity >90% from the serum sample, which is simple and effective to use. Natural Protein A is a cell wall surface protein found in Staphylococcus aureus. Natural Protein G is a cell surface protein isolated from the genus G or C Streptococcus. Both have similar functions and bind most mammalian IgG by interacting primarily with the Fc region of immunoglobulin (Ig), but differ in their binding specificity. Protein A/G MagBeads covalently conjugate both protein A and protein G, providing A wider binding range and higher utility than either protein A or protein G alone. At the same time, this product uses genetically modified proteins A and G, which not only maintain their Ig affinity properties, but also remove the non-major binding domain of the natural protein itself to reduce non-specific binding. This product has a wide range of application, can be used in cell lysis fluid, cell secretory fluid supernatant, serum, animal ascites and other immune antigen samples.
Features
- Low background—little to no nonspecific binding, and no preclearing
- Highly reproducible—uniform beads ensure the most consistent results
- Highly sensitive—Magbeads technology is the most cited method for sensitive applications, such as ChIP and IP, of low abundance proteins
- Fast and easy— no centrifugation or preclearing steps
- Versatile—products for IP, Co-IP, pull-down, and ChIP assays.
Applications
- Immunoprecipitation (IP)
- Purification of antibody
Specifications
| Ligand | rProtein A/G |
| Binding Capacity | ≥50 μg hIgG /mg |
| Particle size | 1 μm |
| Concentration | 10 mg/mL |
| Storage Buffer | PBS, 0.01% Tween-20, 0.02% NaN3 |
Smaller beads, Higher capacity
|
Thermo Dynabeads™ Protein G/Protein A, 10003D/10001D |
Yeasen Protein A/G MagBeads, 36417ES |
|
|
Diameter |
2.8 μm |
1 μm |
|
Concentration |
30 mg/mL |
10 mg/mL |
|
Capacity |
About 8 µg human IgG/mg beads |
About 50µg human IgG /mg beads |
Components
| Components No. | Name | 36417ES03 | 36417ES08 |
| 36417 | rProtein A/G MagBeads (IP Grade) | 1 mL | 5 mL |
Storage
The products should be stably stored at 2~8℃ for 2 year.
Figures
Figure 1. The SDS-PAGE gel electrophoresis results of Rabbit serum purification by Protein A/G MagBeads (IP Grade).
L: sample
L: eluate
36417_Manual_HB230112_EN.pdf
Citations & References:
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