Salt Active UltraNuclease ELISA kit -36703ES

SKU: 36703ES96

Size: 96 T
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Description

Salt Active UltraNuclease is a non-specific endonuclease which derived from Marine microorganisms and is expressed and purified in Escherichia coli (E. coli) by genetically engineered. This nonspecific, recombinant endonuclease has optimum activity at high salt concentration (500mM NaCl), which can improve efficiency and yield in various workflows.

This kit uses the principle of double-antibody sandwich enzyme-linked immunosorbent assay (sandwich ELISA) to detect the residual Salt Active UltraNuclease in samples. First, add the Salt Active UltraNuclease standard and test sample to the Anti-Salt Active UltraNuclease coated microtiter strips (36719-A), then add the diluted biotin labeled Anti-UltraNuclease antibody (36719-C), and finally add Streptavidin-HRP (36719-D) to form an antibody + antigen + antibody-Biotin + SA-HRP complex. Subsequently, add TMB substrate (36719-H) into the complex to observe color reaction after washing the complex. TMB is converted into blue under the catalysis of HRP enzyme and finally converted into yellow in the presence of acid, and the shade of color is positively correlated with the amount of Salt Active UltraNuclease in the sample.

The detection range of this kit is 0.047-3 ng/mL; the lower detection limit is 0.024 ng/mL.

Feature

 

  • Highly sensitive: Detecting as little as 0.024 ng/mL of Salt Active UltraNuclease residuals in in-process and final biological samples.
  • Ensures accuracy: The Salt Active UltraNuclease residuals can be accurately detected with a recovery of 75%-125%.
  • Specificity: Cooperate use with YEASEN Salt Active UltraNuclease and can accurately detect the residuals.
  • Stable: The difference between lot-to-lot is low, the kit property is not impacted under 37℃ for 7 days.
  • Easy signal collection: Using Biotin-Streptavidin system, the signal can be stably enlarged.

Application

  • Residual Salt Active UltraNuclease test in biological products

Specification

Sensitivity

0.024 ng/mL (range 0.047~3 ng/mL)

Assay Time

<4 hours

Assay Principle

Two-site immunoenzymetric assay

Signal Amplification

Biotin-Streptavidin system

Detection Wavelength

450nm-630nm

Components

Components No.

Name

36703ES96

36703-A

Anti-Salt Active UltraNuclease coated microtiter strips

96 T

36703-B*

Standard: Salt Active UltraNuclease

2 vial

36703-C

Detection Antibody:Biotin-conjugated Antibodies

120 μL

36703-D

Streptavidin-HRP

60 μL

36703-E

Dilution Buffer 1

50 mL

36703-F

Wash Buffer Concentrate (20×)

50 mL

36703-G

Dilution Buffer 2

30 mL

36703-H

TMB Substrate

15 mL

36703-I

Stop Solution

10 mL

36703-J

Plate Sealer

5 each

* 1. The stop solution is not provided in this kit, it need to be prepared by user. 1M HCl can be used as the stop solution;

* 2. The Standard (36719-B) is Lyophilized powder.

Storage

This product should be stored at 2°C ~8°C. Unopened product is valid for one year. Once the reagent is opened, it is valid for half a year.

*Upon receipt of the kit, please check whether all components are complete and immediately store them in corresponding condition.

 Figures

    • Precision Display

    A)Repeatability

    Spiked sample

    Intra-Assay

    Duplicates

    Average Conc. (ng/mL)

    CV (%)

    S-1.5 ng/mL

    10

    1.344

    2.97%

    S-0.375 ng/mL

    10

    0.260

    2.27%

    S-0.094 ng/mL

    10

    0.061

    2.56%

    B)Intermediate precision

    Spiked sample

    Inter-Assay

    Duplicates

    Average Conc. (ng/mL)

    CV (%)

    S-1.5 ng/mL

    30

    1.335

    5.70%

    S-0.375 ng/mL

    30

    0.258

    5.59%

    S-0.094 ng/mL

    30

    0.064

    6.82%

    Table 1. The precision of Salt Active UltraNuclease ELISA was assayed as the above scheme

    10 duplicated were tested at 3 concentrations on the standard curve, and all the CV% were lower than 5% (Figure 2A). 3 lot of Salt Active UltraNuclease ELISA kits were tested and the CV% were lower than 10% (Figure 2B).

    • Accuracy Validation

    Dilution factor

    Dilution recovery (%)

    Range of Dilution recovery(%)

    1:2

    92.5

    87.5-98.9

    1:4

    87.0

    79.0-95.2

    1:8

    92.6

    78.9-106.2

    1:16

    96.9

    79.1-118.1

    Spiked samples

    Spike recovery (%)

    Range of Spike recovery(%)

    S-0.75ng/mL

    105.3

    91.0-122.1

    S-0.18ng/mL

    96.6

    86.5-105.5

    Table 2. The accuracy of UCF.ME™ UltraNuclease ELISA was assayed as the following scheme

    The protein sample with high Salt Active UltraNuclease concentration was serial 2 fold diluted and also spiked with 0.75ng/mL and 0.18ng/mL Salt Active UltraNuclease. The dilution recovery and the spike recovery were all within 75%-125% (Figures 3).

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