Description
RNase Inhibitor is a specific ribonuclease (RNase) inhibitor found in the human placenta. Its mechanism of action for specifically inactivating RNase is: it binds to RNase via non-covalent bonds, forming a complex that causes RNase inactivation.
This kit uses the principle of a double antibody sandwich enzyme-linked immunosorbent assay (ELISA) to detect the residual amount of RNase inhibitor. The RNase inhibitor standard and test samples are added to a pre-coated enzyme plate with anti-RNase inhibitor antibodies (36707-A), followed by the addition of a diluted biotin-labeled RNase inhibitor detection antibody (36707-C), and finally Streptavidin-HRP (SA-HRP) (36707-D). This forms the antibody+antigen+antibody-Biotin+SA-HRP complex. After washing, TMB substrate solution (36707-H) is added to develop color.
Under the catalysis of HRP enzyme, TMB is transformed from colorless to blue and, under the action of the stop solution (36707-I), is finally converted into yellow. The intensity of the yellow color is positively correlated with the amount of RNase inhibitor detected in the sample.
Components
|
No. |
Name |
36703ES48 |
36703ES96 |
|
36707-A |
Anti-Murine RNase Inhibitor coated microtiter strips |
48 T |
96 T |
|
36707-B* |
Standard: Murine RNase Inhibitor |
1 vial |
2 vial |
|
36707-C |
Detection Antibody：Biotin-conjugated Antibodies |
60 μL |
120 μL |
|
36707-D |
Streptavidin-HRP |
30 μL |
60 μL |
|
36707-E |
Dilution Buffer 1 |
25 mL |
45 mL |
|
36707-F |
Wash Buffer Concentrate (20×) |
25 mL |
50 mL |
|
36707-G |
Dilution Buffer 2 |
15 mL |
30 mL |
|
36707-H |
TMB Substrate |
8 mL |
15 mL |
|
36707-I |
Stop Solution |
5 mL |
10 mL |
|
36707-J |
Plate Sealer |
3 each |
5 each |
*The standard 36707-B is a lyophilized powder.
Shipping and Storage
Store at 2-8°C. The shelf life is 1 year when unopened, and 6 months after opening.
Upon receipt of the product, please check if all components are complete, and immediately store them at the corresponding storage temperature.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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