Insulin, Human Recombinant -40112ES

SKU: 40112ES60

Size: 100 mg
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Description

Insulin is a double-chain (α, β) polypeptide hormone secreted by islet β cells in the pancreas. It consists of 51 amino acids, of which the α chain contains 21 amino acids and the β chain contains 30 amino acids. The two chains are connected by two pairs of disulfide bonds, and the α chain itself also contains a pair of disulfide bonds. The secretion process of insulin is: the amino terminus of preproinsulin carries a 23-30 amino acid signal peptide. This signal peptide can transfer preproinsulin to the endoplasmic reticulum, and then is immediately cut off to release proinsulin. Proinsulin is composed of insulin β chain, C-peptide and α chain. The released proinsulin is immediately transferred to the Golgi apparatus, where it is packaged and converted into insulin, and is released into the blood together with C-peptide. Insulin is the only protein hormone known in the body that can lower blood sugar. It is not only responsible for regulating the uptake, utilization and storage of glucose, amino acids and fatty acids by cells, but also inhibits the degradation of glycogen, protein and fat. 

This product is a full-length sequence of human insulin expressed in E. coli. The molecular weight is approximately 5800 Da. The structure is the same as that of naturally derived human insulin. It is commonly used as an auxiliary component in cell culture. The common concentration is 1~10 μg/mL.

 

Product Information

 

Catalog Number

40112ES25 / 40112ES60 / 40112ES76 / 40112ES80

Specification

25 mg / 100 mg / 500 mg / 1 g

Source

E.coli.

CAS Number

11061-68-0

Molecular Formula

C257H383N65O77S6

Molecular Weight

5808

Appearance

White powder

Activity

 15 U/mg

Endotoxin

<10 EU/mg

Host Cell Protein

< 10 ng/mg

Microbial Limit

< 300 cfu/g

Purity

95%-105%

Sequence

Gly-Ile-Val-Glu-Gln-Cys-Cys-Thr-Ser-Ile-Cys-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn)-(Phe-Val-  Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe- Tyr-Thr-Pro-Lys-Thr) [Disulfide bonds Cys6/Cys11 in first branch, 7 -7 between branches, 20 -19   between branches

 


 Storage

The product should be stored at -25~-15℃ for 3 years in lyophilized state 

6 months, -25~-15℃ under sterile conditions after reconstitution.

Instructions for Use

Insulin is insoluble in water at neutral pH ,but soluble in 1% dilute acetic acid or 0.5% dilute hydrochloric acid (pH 2-3). It is recommended to prepare a 2 mg/mL stock solution. The stock solution should be aliquoted and stored at -25°C to -15°C to avoid repeated freeze-thaw cycles. Additionally, the stock solution, after being filtered through a low-protein-binding filter or containing suitable preservatives such as 0.1% thimerosal or sodium azide, can be stored at 2°C to 8°C for up to 6 months.

Cautions

1. Avoid aeration and bubble formation during dissolution and filtration as they may cause protein denaturation.

2. Insulin solution should not be autoclaved.

3. This product can also be dissolved in 125 mM NaHCO3. Preparation of an alkaline storage solution is not recommended, as high pH may increase deamidation and protein aggregation.

4. For your safety and health, please wear a lab coat and disposable gloves during operation.

5. This product is for research use only.

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