Description
Ceturegel™ Matrix GFR LDEV-Free Matrix is a growth factors reduced soluble basement membrane preparation extracted from EHS mouse tumors rich in extracellular matrix proteins. Its main components are laminin, type IV collagen, heparan sulfate proteoglycan (HSPG) and nestin. Ceturegel™ GFR Matrix is a low-growth factor Matrix obtained after special treatment, which can effectively ensure the needs of some experimental operations that require low-growth factors, such as experiments related to signaling pathways and growth factors. At room temperature, it aggregates to form a biologically active three-dimensional matrix, which simulates the structure, composition, physical properties and functions of the cell basement membrane in vivo, which is beneficial to the culture and differentiation of cells in vitro. It can be used for cell morphology, biochemical function, migration, Invasion and gene expression studies.
The three-dimensional culture matrix formed by Matrix can promote the adherence and differentiation of epithelial cells, hepatocytes, Sertoli cells, melanoma cells, vascular endothelial cells, thyroid cells, and hair follicle cells. Ceturegel™ Matrix can affect gene expression in three-dimensional cultures of murine hepatocytes and human mammary epithelial cells. At the same time, Ceturegel™ Matrix can be used as a basic scaffold for various tumor cell invasion studies using matrigel, an essential matrix for in vitro and in vivo studies of angiogenesis, and a three-dimensional scaffold for the growth of transplanted tumor cells in in vivo animal models. Ceturegel™ Matrix also supports the regeneration of peripheral nerves and the differentiation of bovine fallopian tube epithelial cells.
YEASEN Matrix is a sterile product, does not contain phenol red, and has a concentration of 8~12 mg/mL, which meets various experimental requirements.
Feature
- High Safety: No LDEV (lactate dehydrogenase elevating virus)
- Multiple Concentration: the concentration range from 8 to 20 mg/ml
- Batch Consistency: Strict process through the whole product life cycle including the production, quality, warehouse etc.
- Low Endotoxin: Endotoxin content <8 EU/ml
- Contamination Control: Adhere to the rigorous residue quality standards of mycoplasmas, bacteria, and fungi
- High yield:＞30L per single batch
- Wide Compatibility: Compatible with almost all types of cell culture medium
Application
- 2D Culture Support
- Migration/Invasion
- Biochemical Research
- 3D & Organoid Culture
- Angiogenesis
- In Vivo Tumor Formation
Specification
| Concentration | 8-12 mg/mL |
| Product Type | Basement Membrane Matrix |
| Endotoxin Level | Low |
| Transportation Conditions | Dry Ice Transportation |
| Product Line | Ceturegel™ |
| Form | Frozen |
| Phenol Red Indicator | None |
| Product specifications | 5/10 mL |
| Species | EHS Mouse Tumors |
| Classification | Low Growth Factor |
| Serum Level | None |
| LDEV Detection | None |
Components
| Components No. | Name | 40186ES08 | 40186ES10 |
| 40186 | Ceturegel™ Matrix GFR，Phenol Red-Free，LDEV-Free | 5 mL | 10 mL |
Shipping and Storage
Dry ice shipping. -15℃ ~ -25℃ storage, valid for two years.
Figures
- Cell application validation
Figure 1. The application validation of Ceturegel™ Matrix were as shown.
The representative image of HepG2 cell stained with crystal violet after invasion (Figure 1A). The image of 3D culture of HepG2 cell for 4 days (Figure 1B). The representative bright field and fluorescent images of HUVEC cell angiogenesis (Figure 1C)
Figure 2. Intestinal organoids formation