Phalloidin is a cyclic heptapeptide toxin derived from the death cap mushroom (Amanita phalloides). It binds to filamentous actin (F-actin) with high affinity (Kd = 20 nM) and does not bind to globular actin (G-actin). It is commonly used to label F-actin in tissue sections, cell cultures, or cell-free systems for qualitative and quantitative analysis. Phalloidin derivatives also bind to actin filaments from both animal and plant sources, including muscle and non-muscle cells, at a stoichiometric ratio of approximately one phalloidin molecule per actin subunit. Non-specific binding is negligible, providing clear distinction between stained and unstained regions. Therefore, phalloidin derivatives are particularly suitable as a substitute for actin antibodies in related research. Additionally, phalloidin derivatives are small, with a diameter of approximately 12-15 Å and a molecular weight of less than 2000 Daltons. Many physiological properties of actin are maintained, such as the ability to interact with actin-binding proteins like myosin, tropomyosin, and DNase I. Phalloidin-labeled filaments can still penetrate solid myosin matrices, and glycerol-extracted muscle fibers can contract after labeling.

Phalloidin binding inhibits the depolymerization of filamentous actin (microfilaments), stabilizing their structure and disrupting the dynamic equilibrium of polymerization and depolymerization. This property reduces the critical concentration (CC) for actin polymerization to less than 1 µg/mL, making it a potent polymerization promoter. Additionally, phalloidin inhibits the ATP hydrolysis activity of F-actin.

This product is TRITC (tetramethylrhodamine isothiocyanate)-labeled phalloidin, which offers high specificity and contrast in staining, superior to actin antibodies. It is suitable for qualitative and quantitative detection of F-actin. The F-actin bound by this product maintains many biological properties of actin itself. Moreover, this product has no species differences and is widely applicable.

We provide TRITC-labeled phalloidin in two forms: lyophilized powder (Cat No.: 40734ES80) and storage solution (Cat No.: 40734ES75, 20 µM concentration). Users can choose according to their needs. The recommended working concentration is 80-200 nM.

Features

Binds selectively to filamentous actin (F-actin).

Phalloidin is not species-specific and exhibits virtually no non-specific staining, providing extremely clear contrast between stained and unstained regions.

It is highly compatible and does not affect the activity of actin.

Applications

The tight and selective binding to F-actin reveals the distribution of microfilament cytoskeleton within the cell. 

Specifications

Components

Shipping and Storage

The product is shipped with ice pack. Can be stored at -15°C ~-25°C in a dark, dry environment for up to one year.

Documents:

Safety Data Sheet

40734ES75-MSDS-CN20250217

40734ES80-MSDS-CN20250217

Manuals

40734_Manual_CN20250217_EN

Citations & References:

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