Description
This product is a Linear Polyethylenimine (PEI) after optimization and transformation, with a concentration of 1 mg/mL. This product is purely chemically synthesized and does not contain animal-derived ingredients. It is particularly suitable for co-transfection of multiple plasmids, for the production of recombinant virus vectors and the instantaneous expression of recombinant proteins. This product has low cytotoxicity, high transfection efficiency, and is compatible with antibiotics. It has high gene expression efficiency in HEK293 and other cells.
Feature
- Small scale and large-scale production of various viruse vectors and transient expression of proteins suitable for adherent and suspension systems
- High transfection efficiency, less plasmid usage and high-cost performance
- Compatible with a variety of commercial medium
- Sufficient capacity and stable batch
Application
- Cell Transfection
Specification
| Physical and chemical Properties | Information |
| Form | Liquid |
| Serum Compatible | Yes |
| Cell Type | Established Cell Lines |
| Sample Type | Plasmid DNA |
| Transfection Technique | PEI-Based Transfection |
Components
| Components No. | Name | 40820ES04 | 40820ES10 | 40820ES60 |
| 40820 | Hieff Trans™ PEI Transfection Reagent | 1.5 mL | 10 mL | 100 mL |
Storage
The product could be stored at 2-8ºC for two years. Do not freeze!
The product should be stored at 2-8℃ for two years. Do not freeze!
Figures
- Quality
| Item |
Specifications |
R&D |
GMP |
|
Clarity |
Colorless liquid |
√ |
√ |
|
Activity |
Transfection efficiency>70% Cell viability>80% |
√ |
√ |
|
Endotoxin |
<0.5 EU/mL |
√ |
√ |
|
Sterility |
Sterile after 14 days |
√ |
√ |
|
Mycoplasma |
Absence |
√ |
√ |
|
pH |
<7.0 |
|
√ |
|
Osmolality |
≤30 mOsm/kg |
|
√ |
|
Impurity distribution |
No residual acetonitrile, methanol, ether, toluene |
|
√ |
- Transfection Efficiency
Figure 1. The transfection efficiency of Hieff Trans™ PEI Transfection Reagent-GMP.
Hieff Trans™ PEI Transfection Reagent-GMP was used to transfect the suspension cell HEK293F cell line and adherent cell HEK293T cell line in a 6-well format. AAV-GFP expression was analyzed 48 hours post-transfection. (the ratio of the plasmid to reagent is 1:1)
- Customer's test data (AAV2)
|
Suspended cell 293F |
|||
|
Sample |
Transfection efficiency |
Cell viability |
Cell count |
|
YEASEN |
100% |
92.00% |
6.80E+6 /ml |
|
P* |
100% |
71.00% |
6.05E+6 /ml |
Culture vessel: 30 mL medium (125 mL shake flask)
Medium: OPM-293CD-05 serum-free medium
Incubation time: 72 h
Plasmid: 60 μg(MCS-GFP : RC2 : pHelper = 1 : 1 : 1 = 20 μg : 20 μg : 20 μg)
Transfection reagent: 120 -180 μL
- Customer's test data (LV)
Cell viability: 98%
Culture vessel: 50 mL medium (250 mL shake flask)
Cell count: 5E+6/mL
Plasmid: 2 mg/L
Transfection reagent: 4-8 ml/L
Incubation time: 48 h