Description
D-luciferin is a common substrate for Luciferase and is widely used throughout biotechnology, especially in vivo imaging Technology. The mechanism of action is that luciferin (substrate) can be oxidized to emit light in the presence of ATP and luciferase. When fluorescein is in excess, the quantum number of light produced is equal to Luciferase concentrations were positively correlated (see figure below). Plasmids carrying luciferase encoding gene (Luc) were transfected into cells and introduced into study animals such as rats and mice.
D-luciferin is also commonly used in in vitro studies, including luciferase and ATP levels analysis; Reporter gene analysis; High-throughput sequencing and various contamination tests. There are three Product form: D-luciferin (free acid), D-luciferin salt (sodium salt and potassium salt). The main difference lies in solubility: water solubility of the former and solubility of the buffer system. They are weakly soluble except in weak bases such as low concentrations of NaOH and KOH solutions. Soluble in methanol and DMSO; The latter can be easily dissolved in water or buffer, easy to use.
Features
- No radiation, almost harmless to living organisms.
- Bioluminescence, no excitation light source.
- So sensitive, you can detect it in a few hundred cells.
- Good penetration, 3-4cm tissue depth can still be detected.
- High signal-to-noise ratio, strong fluorescence signal and good anti-interference.
Applications
- In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.
- To test the effect of the administration on tumor growth or metastasis, the fluorescein substrate can be completely eliminated within 3 hours, without interference to the drug experiment.
- The localization and distribution of foreign cells in animals were detected.
- The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.
- Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo.
Specifications
| English synonym | (S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid potassium salt; D-Luciferin firefly, potassium salt |
| CAS NO. | 115144-35-9 |
| Formula | C11H7N2O3S2K |
| Molecular weight | 318.42 g/mol |
| Appearance | Light yellow powder |
| Solubility | Soluble in water(60 mg/mL) |
Components
| Components No. | Name | 40902ES01 | 40902ES02 | 40902ES03 | 40902ES08 |
| 40902 | D-Luciferin, Potassium Salt | 100mg | 500 mg | 1g | 5g |
Shipping and Storage
The product is shipped with dry ice and can be stored at -15℃ ~ -25℃ for 1 year.
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