Description
Coelenterazine h is a dehydroxylated derivative of native coelenterazine and serves as a substrate for Renilla luciferase (Rluc) as well as a cofactor for jellyfish luminescent protein. Its luminescence intensity is more than 10 times higher than that of native coelenterazine, making it suitable for reporter gene analysis. Additionally, it can be used for the detection of intracellular calcium ion concentrations, gene reporter analysis, bioluminescence resonance energy transfer (BRET) studies, ELISA, high-throughput screening (HTS), and chemiluminescent detection of reactive oxygen species (ROS) levels in tissues or cells.
Features
- No radiation, almost harmless to living organisms.
- Bioluminescence, no excitation light source.
- So sensitive, you can detect it in a few hundred cells.
- Good penetration, 3-4 cm tissue depth can still be detected.
- High signal-to-noise ratio, strong fluorescence signal and good anti-interference.
Applications
- In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.
- To test the effect of the administration on tumor growth or metastasis.
- The localization and distribution of foreign cells in animals were detected.
- The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.
- Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo.
Specifications
|
English synonym |
Coelenterazine h |
|
CAS NO. |
50909-86-9 |
|
Formula |
C26H21N3O2 |
|
Molecular weight |
407.5 g/mol |
|
Appearance |
Yellow powder |
|
Solubility |
Soluble in methanol and ethanol, insoluble in DMSO |
|
Purity |
≥ 99% |
Components
|
Components No. |
Name |
40905ES02 |
40905ES03 |
40906ES08 |
40906ES10 |
|
40906 |
Coelenterazine 400a |
1×500 μg |
2×500 μg |
5 mg |
5 mg×2 |
Shipping and Storage
-20℃ keep away from light and dry storage, preferably stored in an inert gas environment, avoid contact with air; long-term storage at -70℃. Valid for 2 years.
Figures
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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