Description
Coelenterazine f is a derivative of native coelenterazine and serves as a substrate for aequorin (jellyfish luminescent protein). The structural difference between coelenterazine f and native coelenterazine lies in the substitution of the hydroxyl group on the phenol ring at the R-1 position with a fluorine (F) atom.
When coelenterazine f and aequorin are oxidized, they are activated to form a metastable complex of coelenterazine. The formation of this complex occurs very rapidly, whereas the formation of the native coelenterazine–aequorin complex is much slower (only 40% of native coelenterazine reacts within 60 minutes). Compared to the total photon energy produced by the native coelenterazine–aequorin complex, this complex generates only 80% of the photon energy.
Upon contact with Ca2+, coelenterazine f can rapidly and efficiently emit light, with an energy output that is 20 times stronger than that of native coelenterazine. Additionally, coelenterazine f exhibits the best cell permeability. When extremely high Ca2+detection sensitivity is required for experiments involving aequorin regeneration, this substrate is highly recommended.
Features
- No radiation, almost harmless to living organisms.
- Bioluminescence, no excitation light source.
- So sensitive, you can detect it in a few hundred cells.
- Good penetration, 3-4cm tissue depth can still be detected.
- High signal-to-noise ratio, strong fluorescence signal and good anti-interference.
Applications
- In the tumorigenesis experiment in nude mice, the tumor growth was observed without invasion in real time, without tumor stripping measurement.
- To test the effect of the administration on tumor growth or metastasis.
- The localization and distribution of foreign cells in animals were detected.
- The target gene or promoter of the target gene is fused to the luciferase gene to detect changes in gene expression during drug treatment or disease progression.
- Monitoring stem cell transplantation, survival and proliferation; Trace the distribution and migration of stem cells in vivo.
Specifications
|
English synonym |
Coelenterazine f |
|
CAS NO. |
123437-16-1 |
|
Formula |
C26H20N3O2 |
|
Molecular weight |
425.46 g/mol |
|
Appearance |
Yellow to brown powder |
|
Solubility |
Soluble in methanol and ethanol, insoluble in DMSO |
|
Purity |
≥ 95% |
Components
|
Components No. |
Name |
40908ES02 |
40908ES03 |
|
40906 |
Coelenterazine f |
1×500 μg |
2×500 μg |
Shipping and Storage
-20℃ keep away from light and dry storage, preferably stored in an inert gas environment, avoid contact with air; long-term storage at -70℃. Valid for 2 years.
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