Ampicillin is a β-lactam antibiotic within the penicillin family. As a member of this family, Ampicillin is susceptible to β-lactamase, which hydrolyzes the β-lactam ring. This broad-spectrum antibiotic is effective against gram-positive, gram-negative bacteria, and anaerobic bacteria. As an antibiotic, Ampicillin binds to penicillin-binding proteins (PBPs), acting as a structural analog of acyl-D-alanyl-D-alanine, acylating the transpeptidase enzyme, and thereby preventing the cross-linking of the peptidoglycan of the cell wall necessary for the growth of the bacterium. Ampicillin in the form of sodium salt from Yeasen, as a selective agent in E.coli culture, is widely used to prepare LB medium.

As a selective agent in E.coli culture, Ampicillin is widely used to prepare LB medium. The minimum inhibitory concentration (MIC) of ampicillin against Gram-negative bacteria and Gram-positive bacteria were 0.03-3 μg/mL and 0.02-1.5 μg/mL, respectively. Agar or culture medium for autoclave should be cooled to 45-50°C before adding ampicillin solution. A culture medium containing ampicillin can be stored at 2-8°C for 2 weeks, and on 37°C for approximately 3 days.

This product is ampicillin sodium salt. The recommended concentration for cell screening is 50-100 μg/mL.

For other antibiotics, please refer to the related topic of Antibiotic User Manual.

Features

Potency (Anhydrous)：845-988 mcg/mg

Standardized production, using factory mass production mode

Applications

Widely used to prepare LB medium

Cell culture

Specifications

CAS NO.	69-52-3
Molecular Fomular	C16H18N3NaO4S
Molecular Weight	371.39 g/mol
Appearance	White to off-white powder
Potency (Anhydrous)	845-988 mcg/mg
Solubility	Soluble in water: ≥ 50 mg/mL
Structure

Components

Shipping and Storage

The Ampicillin, Sodium Salt products be stored at 2～8℃ for 3 years. 

Documents:

Safety Data Sheet

60203_MSDS_HB250210_EN.pdf

Manuals

60203_Manual_HB250210_EN.pdf