Kanamycin sulfate is an aminoglycoside antibiotic that has inhibitory effects on both Gram-positive and Gram-negative bacteria, as well as mycoplasmas. Its mechanism of action targets the binding to the bacterial 70S ribosomal subunits, thereby inhibiting ribosomal translocation and causing misreading, which further interferes with protein synthesis. Kanamycin sulfate can be used in cell culture to suppress bacterial contamination. In molecular biology research, it is commonly used for selective screening of bacterial colonies that have successfully been transformed with the kanamycin resistance gene (kan gene); it is also often used in Agrobacterium-mediated transformation experiments for the selective screening of plant tissues carrying the npt II (APH(3')-II) gene.

This product is easily soluble in water (100 mg/mL), and it should be filtered and sterilized before use. The recommended working concentration is 30-100 µg/mL.

For other antibiotics, please refer to the related topic of Antibiotic User Manual.

Features

Potency (dry basis) ≥750 µg/mg

Standardized production, using factory mass production mode

Applications

Cell culture

Commonly used to selectively screen bacterial colonies

Frequently used in Agrobacterium-mediated transformation experiments to selectively screen plant tissues carrying the npt II (APH3) gene

Specifications

CAS NO.	25389-94-0
Molecular Fomular	C18H36N4O11·H2O4S
Molecular Weight	582.58 g/mol
Appearance	White or off-white
Potency (dry basis)	≥ 750 µg/mg
Structure

Components

Shipping and Storage

The Kanamycin Sulfate products be stored at room temperature for 3 years.

Documents:

Safety Data Sheet

60206_MSDS_HB250210_EN.pdf

Manuals

60206_Manual_HB250210_EN.pdf