Streptozocin _ 60256ES

Streptozocin (STZ) is an antitumor antibiotic produced from a certain Streptomyces Aureobasidium pullulans, and can also be synthesized artificially. Often used to treat pancreatic cancer. At the same time, STZ selectively destroys islet β cells of certain species of animals, and can induce diabetes in many animals. Generally, rats and mice are used to make animal models.

Diabetes modeling is related to the dose of STZ injection: when a large dose is injected, it can directly cause extensive destruction of islet β cells, which can cause type I diabetes model; Peripheral tissue is not sensitive to insulin, and high-calorie feed is fed at the same time, and the combination of the two induces pathological and physiological changes that are close to the animal model of human type Ⅱ diabetes.

In addition, STZ has been widely studied in anti-leukemia, DNA methylation, anti-nephritis and so on.

Specifications

Storage

This product should be stored at -25～-15℃ for 2 years.

【Notes】This product is hygroscopic and prone to moisture absorption. If multiple samplings and weighings are required, it is essential to avoid leaving it open for extended periods to prevent moisture exposure. The product becomes ineffective after being exposed to moisture for 30 minutes, which is analogous to the requirement for rapid injection during modeling, indicating that its aqueous solution is unstable. If multiple weighings are necessary, it is crucial to operate and store the product in strict accordance with the principles of moisture avoidance.

Instructions

Take a diabetes model as an example to illustrate the use of STZ.

1. Prepare citrate buffer

Solution A: Weigh 2.1 g of citric acid (MW: 210.14) and add it to 100 mL of double distilled water to prepare Solution A.

Solution B: 2.94 g of sodium citrate (MW: 294.10) was added to 100 mL of double distilled water to prepare solution B.

Mix the A and B solutions in a certain ratio (1:1.32 or 1:1), measure the pH value, and adjust the pH within the range of 4.2-4.5, that is, the citric acid buffer for STZ preparation.

2. Preparation before injection

Before preparing STZ injection, place STZ in a dry sterilized bottle, wrap it with tin foil, and place it in an ice bath with citric acid buffer to pre-cool, and take it to the animal room for use.

3. Preparation of injections

Rats were weighed after an overnight fast. Rats were grouped to dissolve STZ by group. 1% STZ injection was prepared in citrate buffer based on fasting body weight. If the follow-up injection operation is not proficient, do not dissolve STZ at one time.

【Notes】STZ is easy to be deactivated, and STZ needs to be dried and protected from light after being weighed quickly. It is recommended to use dry aluminum foil (or tin foil) paper.

4. Injection

Intraperitoneal injection or tail vein injection, if the injection technique is not skilled, the two groups should be injected alternately, and the injection should be completed within 30 minutes. 【Notes】 Most injections require a bolus injection.

For detailed steps, please refer to the special topic:Streptozotocin (STZ) Induced Diabetes Mellitus Animal Model.

Notes

1. STZ is unstable when exposed to moisture. If multiple weighings are required, it is essential to operate and store the substance strictly according to principles that avoid moisture. The operating environment, containers, and tools for portioning must all remain dry.

2. The injection solution should only be prepared immediately before administration, as the STZ aqueous solution is highly unstable.

3. This product is for research use only and is prohibited for use on humans.

Manuals