Description
Interleukin-18 (IL-18), also known as IL-1F4, is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. It is expressed as a 24 kDa precursor by endothelial and epithelial cells, keratinocytes, gamma δ T cells, and phagocytes. Mature human IL-18 shares approximately 63% amino acid sequence identity with mouse and rat IL-18. IL-18 activation is induced by infection or tissue damage and leads to disease pathology in chronic inflammation. In the presence of IL-12 or IL-15, IL-18 enhances anti-viral Th1 immune responses by inducing IFN-gamma production and the cytolytic activity of CD8+ T cells and NK cells. IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells. IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst. In the absence of IL-12 or IL-15, IL-18 promotes production of the Th2 cytokines IL-4 and IL-13 by CD4+ T cells and basophils. IL-18 induces the antigen-independent production of IL-17 by gamma δ T cells and CD4+ T cells. IL-18 also promotes myeloid dendritic cell maturation and triggers neutrophil respiratory burst. In cancer, IL-18 has shown a variety of activities, including enhancing anti-tumor immunity, inhibiting or promoting angiogenesis, and promoting tumor cell metastasis.
Product Properties
|
Synonyms |
IL-1F4 |
|
Accession |
Q14116 |
|
GeneID |
3606 |
|
Source |
E.coli-derived Human IL-11, Tyr37-Asp193. |
|
Molecular Weight |
Approximately 18.2 kDa. |
|
AA Sequence |
YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIIS MYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDII FFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIM FTVQNED |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 95% by SDS-PAGE analyses. |
|
Biological Activity |
Test in processing. |
|
Endotoxin |
Less than 0.1 EU/μg of rHuIL-18 as determined by LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered solution in PBS, pH 7.0. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
Payment & Security
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