Description
Interleukin-12 (IL-12), also known as NKSF, TCMF, CLMF and TSF, is a heterodimeric cytokine composed of p35 and p40 subunits. It is produced by monocytes, macrophages, B cells and dendritic cells in response to bacterial lipopolysaccharides and intracellular pathogens. IL-12 signals throughtheIL-12 receptor complex, which is comprised of IL-12 Rβ1 and IL-12 Rβ2. IL-12 induces the proliferation and activation of hematopoietic stem cells, natural killer cells and T- cells. It is indispensible during the development of Th1 cells, leading to the production of IFN-gamma and IL-2.
Product Properties
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Synonyms |
IL-12a ; IL-12b ; IL-12p35 ; IL-12p40 ; IL12p40 ; IL12a ; p35 ; p40 ;IL12 |
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Accession |
p35: P43431 p40: P43432 |
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Source |
CHO-derived Mouse IL-12 protein, IL-12 p35 (Arg23-Ser215) & IL-12 p40 (Met23-Ser335). |
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Molecular Weight |
27~29 kDa (p35), 45~50 kDa (p40), on SDS-PAGE under reducing conditions. |
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Tag |
None |
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Purity |
> 95% by SDS-PAGE. |
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Biological Activity |
ED50 < 0.15 ng/ml, measured in a cell proliferation assay using 2D6 cells. |
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Endotoxin |
< 0.2 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized after extensive dialysis against PBS. |
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Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -80℃ for 1 year.
1 week, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Avoid repeated freeze-thaw cycles.
2.For your safety and health, please wear lab coats and disposable gloves for operation.
3.For research use only.
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