Description
IL-3, also known as Interleukin-3, is a cytokine that is produced by activated T cells and mast cells. It plays a crucial role in regulating the growth, differentiation, and function of various cell types in the immune system. human IL-3 is an important cytokine that regulates the growth, differentiation, and function of immune cells. Its study contributes to our understanding of immune system regulation and its potential applications in immunotherapy and disease treatment.
Recombinant human IL-3 is a 15.0 kDa recombinant protein consisting of 133 amino acid residues. This product is expressed in E. coli and provided in a lyophilized powder form, featuring high activity, high purity, low endotoxin levels, and no tag.
Product Properties
|
Synonyms |
Interleukin-3; IL-3; Hematopoietic growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor |
|
Source |
E.coli. |
|
Sequence |
Ala20-Phe152. |
|
Molecular Weight |
Approximately 15.08 kDa. |
|
Accession |
P08700 |
|
Tag |
No Tag |
|
Biological Activity |
The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
＜ 0.01EU/μg protein as determined by LAL method. |
|
Sterility |
Negative. |
|
Mycoplasma |
Negative. |
|
Vitro Virus |
Negative. |
|
Host Cell Protein |
Less than 0.5 ng/ug when tested by ELISA. |
|
Host Cell DNA |
Less than 20 ng/mg when tested by qPCR. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0. |
|
Physical Appearance |
Sterile filtered white lyophilized (freeze-dried) powder. |
Storage Conditions
- Store at -25°C to -15°C for up to 3 years.
- After reconstitution, store at 2°C to 8°C for up to 30 days.
- After reconstitution, store at -85°C to -65°C for up to 3 months.
Reconstitution Method
It is recommended to reconstitute Human IL-3 in sterile PBS to a concentration of 0.1–1.0 mg/mL. Avoid repeated freeze-thaw cycles as much as possible.
Cautions
- For your safety and health, please wear a lab coat and disposable gloves during operation.
- This product is for research use only.
Figures
SDS- PAGE
Human IL-3 on SDS-PAGE under reduced condition.
The purity is greater than 95%.
Cell Based Assay
The ED50 as determined by a cell proliferation assay using human TF-1 cells
is less than 0.1 ng/mL, corresponding to a specific activity of > 1.7 × 106 IU/mg.
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