Description
BAFF Receptor (BAFFR), a member of the TNFR superfamily, is highly expressed in the spleen, lymph nodes, and resting B cells, and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFFR is a type III transmembrane protein that binds with high specificity to BAFF (TNFSF13B). ?BAFFR/BAFF signaling plays a critical role in B cell survival and maturation. ?Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B?cells. It is also expressed at lower levels in activated B cell, in resting CD4+?T?cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA
BAFF Receptor (BAFFR), a member of the TNFR superfamily, is highly expressed in the spleen, lymph nodes, and resting B cells, and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFFR is a type III transmembrane protein that binds with high specificity to BAFF (TNFSF13B). BAFFR/BAFF signaling plays a critical role in B cell survival and maturation. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cell, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells. Similarly, A/WySnJ mice that are defective in BAFF-R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses.
Product Properties
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Synonyms |
BAFFR, TNFRSF13C |
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Accession |
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GeneID |
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Source |
E.coli-derived Human B cell Activating Factor Rececptor protein,Met1-Gly76,with an N-terminal Met |
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Molecular Weight |
Approximately 7.8 kDa |
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AA Sequence |
MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPG |
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Tag |
None |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 95% by SDS-PAGE and HPLC analyses. |
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Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by its ability to block BAFF induced mouse splenocyte survival is 1.0-5.0 μg/ml in the presence of 1.0 μg/ml of rHuBAFF. |
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Endotoxin |
< 1 EU/μg of protein as determined by LAL method. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 8.0, 500 mM NaCl. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.
- or TACI-deficient mice have no major defect in B-cell development. While the function of BCMA is not defined, TACI has been shown to control B-cell homeostasis and T-cell-independent immune responses.
?
Product?Properties
|
Synonyms |
BAFFR, TNFRSF13C |
|
Accession |
|
|
GeneID |
|
|
Source |
E.coli-derived Human B cell Activating Factor Rececptor protein,Met1-Gly76,with an N-terminal Met |
|
Molecular Weight |
Approximately 7.8 kDa |
|
AA Sequence |
MRRGPRSLRG RDAPAPTPCV PAECFDLLVR HCVACGLLRT PRPKPAGASS PAPRTALQPQ ESVGAGAGEA ALPLPG |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 95% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50?as determined by its ability to block BAFF induced mouse splenocyte survival is 1.0-5.0 ¦Ìg/ml in the presence of 1.0 ¦Ìg/ml of rHuBAFF. |
|
Endotoxin |
< 1 EU/¦Ìg of protein as determined by LAL method. |
|
Formulation |
Lyophilized from a 0.2 ¦Ìm filtered concentrated solution in 20 mM PB, pH 8.0, 500 mM NaCl. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ¡Ü -20¡ãC. Further dilutions should be made in appropriate buffered solutions. |
?
Shipping and Storage
The products are shipped with ice pack and can be stored at?-20¡æ to -80¡æ?for?1?year.
Recommend to aliquot the protein into smaller quantities when first used?and avoid repeated freeze-thaw?cycles.
?
Cautions
1.?Avoid repeated freeze-thaw cycles.
2.?For your safety and health, please wear lab coats and disposable gloves for operation.
3.?For research use only.