Description
CXCL2, also named MIP-2, is belonging to the CXC chemokine family. The protein sequence of mouse CXCL2 shows approximately 63% identity to that of mouse KC, another mouse alpha chemokine whose expression is induced by PDGF. The amino acid sequence of mouse CXCL2 is 60 % identical to human CXCL2. This chemokine is secreted from a mouse macrophage cell line. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is a part of chemokine superfamily, which encodes secretory proteins involved in immune regulation and inflammation. CXCL2, a critical chemokine for neutrophils, has been shown to be produced in the rat intestine in response to platelet-activating factor (PAF) and to mediate intestinal inflammation and injury.
Product Properties
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Synonyms |
chemokine (C-X-C motif) ligand 2, CINC-2a, CINC3, C-X-C Motif Chemokine 2, CXCL2, GRO beta, GRO2, GROB, Growth-regulated protein beta, Macrophage inflammatory protein 2-alpha, MGSA beta, MGSA-beta, MIP2, SCYB2 |
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Accession |
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GeneID |
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Source |
E.coli-derived mouse MIP-2/CXCL2 protein, Ala28-Asn100. |
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Molecular Weight |
Approximately 7.8 kDa. |
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AA Sequence |
AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN |
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Tag |
None |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
>97% by SDS-PAGE and HPLC analyses. |
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Biological Activity |
The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 1.0-10 ng/ml. Fully biologically active when compared to standard. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!