Description
CXCL4, also called PF4, is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand. Mature mouse CXCL4 shares 76%, 88%, 64%, 64% and 63% amino acid sequence identity with human, rat, ovine, porcine and bovine CXCL4, respectively. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. CXCL4 can be antiproliferative and antiangiogenic, at least in part via interfering with FGF-2 and VEGF heparin binding and thus inhibiting their signaling. Tumor tissue revealed up-regulation of CXCL14 in cancer-associated fibroblasts of a majority of prostate cancer. Fibroblasts overexpressing CXCL14 promoted the growth of prostate cancer xenografts, and increased tumor angiogenesis and macrophage infiltration.
Product Properties
|
Synonyms |
chemokine (C-X-C motif) ligand 4, C-X-C motif chemokine 4, CXCL4, CXCL4iroplact, Iroplact, MGC138298, Oncostatin-A, PF4, platelet factor 4 |
|
Accession |
|
|
GeneID |
|
|
Source |
E.coli-derived mouse PF-4/CXCL4 protein, Val30-Ser105. |
|
Molecular Weight |
Approximately 8.2 kDa. |
|
AA Sequence |
VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
>97% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml. Fully biologically active when compared to standard. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.