Description
CXCL12 is belonging to the CXC chemokine family. SDF1 alpha and SDF1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 is highly conserved between species, mouse CXCL12α shares approximately 93% amino acid sequence identity with human CXCL12α. CXCL12 is strongly chemotactic for T-lymphocytes, monocytes, but not neutrophils. Contrast to SDF-1β, SDF-1α is shorter by four amino acids at the C-terminal tail.
Product Properties
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Synonyms |
CXCL12 chemokine (C-X-C motif) ligand 12, CXCL12/SDF-1 alpha, IRH, PBSF, SCYB12, SDF1 alpha, SDF1, SDF1a, SDF1B, TLSF, TPAR1 |
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Accession |
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GeneID |
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Source |
E.coli-derived mouse SDF-1α/CXCL12α protein, Lys22-Lys89. |
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Molecular Weight |
Approximately 8.0 kDa. |
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AA Sequence |
KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK |
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Tag |
None |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
>97% by SDS-PAGE and HPLC analyses. |
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Biological Activity |
The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/mL. Fully biologically active when compared to standard. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ for 1 year.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
- Avoid repeated freeze-thaw cycles.
- For your safety and health, please wear lab coats and disposable gloves for operation.
- For research use only!
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