Description
The MCP proteins are members of the CC chemokine family that signal through CCR2 and, with the exception of MCP-1, other CCR receptors. The MCP proteins chemoattract and activate monocytes, activated Tcells, basophils, NK cells, and immature dendritic cells. The MCP family cross-reacts across species. Recombinant Mouse MCP-3 is an 8.5 kDa protein containing 74 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.
Product Properties
|
Synonyms |
Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC |
|
Accession |
Q03366 |
|
GeneID |
20306 |
|
Source |
E.coli-derived Mouse MCP-3 protein,Gln24-Pro97. |
|
Molecular Weight |
Approximately 8.5 kDa. |
|
AA Sequence |
QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP |
|
Tag |
None |
|
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
> 95% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/mL. |
|
Endotoxin |
<1 EU/μg of protein as determined by LAL method. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 2× PBS, pH 7.4. |
|
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only.