Description
Product Properties
Synonyms |
SCYA25, Ckb15 |
Uniprot No. |
035903 |
Source |
Mouse CCL25 is expressed from E.coli without tag. |
Molecular Weight |
Approximately 14.1 kDa, a single, non-glycosylated polypeptide chain containing 121 amino acids. |
AA Sequence |
QGAFEDCCLG YQHRIKWNVL RHARNYHQQE VSGSCNLRAV RFYFRQKVVC GNPEDMNVKR AIRILTARKR LVHWKSASDS QTERKKSNHM KSKVENPNST SVRSATLGHP RMVMMPRKTN N |
Purity |
> 95 % by SDS-PAGE and HPLC analyses. |
Biological Activity |
The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-50 ng/ml. |
Endotoxin |
< 1 EU per 1μg of the protein by the LAL method. |
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
1 month, 2 ~8 °C under sterile conditions after reconstitution.
3-6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
- Please operate with lab coats and disposable gloves,for your safety.
- This product is for research use only.