Description
Basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor (FGF) family. It is a growth factor and signaling protein encoded by the FGF2 gene. FGF family members have a wide range of mitogenic and cell survival activities and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. In normal tissues, bFGF is present in the basement membrane and the extracellular matrix under the endothelium of the blood vessels. bFGF can stimulate and regulate the differentiation and proliferation of various cells originating from the mesoderm and neuroectoderm, such as vascular endothelial cells, epithelial cells, myoblasts, osteoblasts and glial cells, and plays an important role in embryonic development and tissue healing. bFGF/FGF-2 is an essential component for maintaining pluripotency in all embryonic and induced pluripotent stem cell culture media.
This product is expressed by Escherichia coli and provided in liquid form with high activity, high purity, low endotoxin and no labeling.
Performance parameters
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Synonyms |
bFGF, FGF basic, FGF2, FGF-2, fibroblast growth factor 2 (basic), HBGF-2, Prostatropin. |
|
Source |
Human bFGF Protein expressed in E.coli. |
|
Tag |
No Tags. |
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Sequence |
Ala 144 - Ser 288. |
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Molecular Weight |
Approximately 16.29 kDa. |
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Accession |
P09038. |
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Biological Activity |
The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Endotoxin |
＜0.01EU/μg protein as determined by LAL method. |
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Sterility |
Negative. |
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Mycoplasma |
Negative. |
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Vitro Virus |
Negative. |
|
Host Cell Protein |
Less than 0.5 ng/ug when tested by ELISA. |
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Host Cell DNA |
Less than 20 ng/mg when tested by qPCR. |
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Formulation |
Liquuid from 0.22 μm filtered solution in PBS. |
|
Physical Appearance |
Liquid. |
Storage conditions
Store at -85~-65℃, valid for 2 years.
Precautions
1. For your safety and health, please wear lab coat and disposable gloves when operating.
2. This product is for scientific research purposes only.
Figure
SDS-PAGE
Human FGF basic/FGF2/bFGF on SDS-PAGE under reduced condition. The purity is greater than 95%.
SEC-HPLC
The purity of Human bFGF is greater than 95% as determined by SEC-HPLC.
Cell Based Assay
The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg.
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