Description
TGFβ3 is a member of the TGF-β superfamily subgroup, defined by its structural and functional similarities. TGF-β3, along with β1 and β2, acts as a cellular switch to regulate immune function, cell proliferation, and epithelial-mesenchymal transition. The human TGF-β3 cDNA encodes a 412 amino acid precursor, which includes a 20 amino acid signal peptide and a 392 amino acid protein. Mature human TGF-β3 shares 100%, 99%, and 98% homology with mouse/dog/horse, rat, and pig TGF-β3, respectively. TGF-β3 exhibits high affinity for TGF-βRII (a type II serine/threonine kinase receptor). This receptor phosphorylates and activates the type I serine/threonine kinase receptors TGF-βRI or ALK-1 to regulate transcription via Smad phosphorylation.
The product features high activity, high purity, and low endotoxin levels. Its biological activity and batch-to-batch consistency are ensured through validation and testing for biological activity, endotoxin levels, and SDS-PAGE. Our product is offered in carrier-free form, making it highly suitable for cell culture, ELISA, or Western blot standard research and production.
Product Properties:
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Aliases: TGF-β3; ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor beta 3
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Uniprot No.: P10600
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Expression System and Expression Range: E.coli-derived Human TGF-β3, Ala301-Ser412 with a His tag at the C-terminus
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Molecular Weight: Approximately 12 kDa
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Amino Acid Sequence: MALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS
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Appearance: Sterile Filtered White lyophilized (freeze-dried) powder
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Purity: 98% as determined by SDS-PAGE
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Biological Activity: Assayed by its ability to inhibit IL-4-induced proliferation in HT-2 cells. The ED50 for this effect is <50 pg/mL. The specific activity of recombinant human TGF beta 3 is > 2 x 107 IU/mg.
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Endotoxin: < 0.1 EU per 1μg of the protein by the LAL method
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Formulation: Lyophilized from a 0.2 μm filtered concentrated solution in 1×PBS, pH 3.5
Usage:
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Reconstitution of the lyophilized powder is recommended with sterile 10mM HCl, at a concentration not less than 100 μg/mL, and incubated for at least 20 minutes for complete dissolution. (Avoid vortex shaking)
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The reconstituted solution can be further diluted and aliquoted, with a shelf life of 1 month at 2-8°C and 3 months at -20°C to -80°C; avoid repeated freeze-thaw cycles.
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When further diluting and aliquoting the reconstituted solution, a certain amount of carrier protein (0.1% BSA, 10% FBS, or 5% HSA) should be added. For serum-free experiments, a 5% trehalose solution can be used as a carrier.
Transportation and Storage:
Transportation with ice packs. Store at -20°C, with a shelf life of one year. It is advisable to aliquot and freeze upon first use to avoid repeated freeze-thaw cycles.
Cautions:
For your safety and health, wear lab coats and disposable gloves when handling. This product is for research use only.