Description
Activin A is a member of the TGF-β family and has a wide range of biological activities, including regulation of cell proliferation and differentiation and promotion of neuronal survival. Elevated activin A levels in human colorectal and postmenopausal women have been associated with colorectal and breast cancer, respectively. The biological activity of activin A can be neutralized by inhibin and the diffusible TGF-β antagonist follicle suppressor. Activin A binds to two forms of activin receptor type I (action RI-A and action RI-B) and two forms (action RII-A and action RII-B). Activins are homodimers or heterodimers of different β-subunits. They are produced as precursor proteins with an amino-terminal prepeptide that is cleaved to release a C-terminal bioactive ligand.
This product is supplied as a lyophilized powder derived from HEK293 cells and is highly active and pure. Recombinant Human/Mouse/Rat Activin A is a 26.0 kDa disulfide bonded homodimer of two βA chains, each containing 116 amino acid residues.
Specification
|
Source |
Human Activin A Protein is expressed from HEK293 without tag. It contains Gly311-Ser426. |
|
Accession |
P08476 |
|
Molecular Weight |
The protein has a predicted MW of 12.97 kDa. Due to glycosylation, the protein migrates to 14-15 kDa under reduced (R) condition, and 23-25 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
|
Endotoxin |
Less than 0.1 EU per μg by the LAL method. |
|
Purity |
> 95% as determined by Tris-Bis PAGE |
|
Activity |
Measured by its ability to inhibit proliferation of MPC-11 cells. The ED50 for this effect is < 7 ng/mL. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in 4 mM HCI. Normally 8% trehalose is added as protectant before lyophilization. |
Storage
-25 ~ -15 ° C storage, 1 year expiration date after receipt.
After re-solution, store at 2 ~8 °C, 7 days expiration date. After reconstitution, store at -85~-65 °C, 3 months expiration date.
It is recommended to aliquot the protein for the first use to avoid repeated freeze-thaw cycles.
Compounding Methods
Centrifuge before opening the cap. Use 4 mM HCl to reconstitute to a concentration of not less than 0.1 mg/mL.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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