Description
NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system.NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4).
Product Properties
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Synonyms |
beta Nerve Growth Factor; Ngfb |
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Uniprot No. |
NP_001106168.1 |
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Source |
Recombinant Mouse NGF Protein is expressed from CHO Stable Cells Cells with an initial Met at the C-terminus. It contains Ser 122-Gly 241. |
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Molecular Weight |
The recombinant mouse NGF has a calculated molecular mass of 13.5 kDa as estimated in SDS-PAGE under reducing conditions. |
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Biological Activity |
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 1-6 ng/mL. |
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Purity |
> 95% as determined by SDS-PAGE. |
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Endotoxin |
<1 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from sterile 150mM NaCl, 50mM NaAc, pH 5.5.Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. |
Shipping and Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1.Please operate with lab coats and disposable gloves,for your safety.
2.This product is for research use only.