Description
Bcl-xL is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Bcl-xL is an antiapoptotic member that prevents release of cytochrome c from the mitochondria intermembrane space into the cytosol. Bcl-xL is present on the outer mitochondrial membrane and is also found on other membranes in some cell types. Natural Bcl-xL contains a carboxylterminal mitochondria targeting sequence. Recombinant Bcl-xL missing the mitochondrial targeting sequence maintains its ability to neutralize proapoptotic Bcl-2 family members. Neutralization by Bcl-xL appears to be through binding the BH3 region of proapoptotic Bcl-2 family members. This activity does not require the mitochondrial targeting sequence.
Product Properties
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Synonyms |
Apoptosis regulator Bcl-X; bcl2-L-1 |
|
Accession |
Q64373 |
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GeneID |
12048 |
|
Source |
E.coli-derived Human Mouse Bcl-xL, Ser2-Arg212, with an N-terminal Met. |
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Molecular Weight |
Approximately 23.7 kDa. |
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AA Sequence |
SQSNRELVVD FLSYKLSQKG YSWSQFSDVE ENRTEAPEET EAERETPSAI NGNPSWHLAD SPAVNGATGH SSSLDAREVI PMAAVKQALR EAGDEFELRY RRAFSDLTSQ LHITPGTAYQ SFEQVVNELF RDGVNWGRIV AFFSFGGALC VESVDKEMQV LVSRIASWMA TYLNDHLEPW IQENGGWDTF VDLYGNNAAA ESRKGQERFN R |
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Tag |
None |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 97% by SDS-PAGE and HPLC analyses. |
|
Biological Activity |
Test in Process. |
|
Endotoxin |
Less than 0.1 EU/µg of rMuBcl-xL as determined by LAL method. |
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Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4, 5% Trehalose. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
Shipping and Storage
The products are shipped with ice pack and can be stored at -20℃ to -80℃ for 1 year.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Cautions
1. Avoid repeated freeze-thaw cycles.
2. For your safety and health, please wear lab coats and disposable gloves for operation.
3. For research use only!
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