Description
Mesothelin, also known as CAK1 and ERC, is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation. This recombinant human Mesothelin lacks the 8 aa insertion, and within aa 296-580 it shares 59% sequence identity with mouse and rat Mesothelin. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is upregulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression.
Specifications
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Synonyms |
MSLN, CAK1, Mesothelin, MPF, MPFSMRP, SMR |
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Uniprot No. |
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Source |
HEK293 Cells-derived Human MSLN/Mesothelin Protein, Glu296-Asp580 with hFc tag at the C-terminal |
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Molecular Weight |
Approximately 59.0 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Tris-Bis PAGE result. |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC |
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Activity |
1.ELISA Data: Immobilized Human CA125, His Tag at 2 μg/mL (100 μL/Well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 2.8 μg/mL determined by ELISA. 2.ELISA Data: Immobilized Human MSLN at 0.5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 29.2 ng/mL determined by ELISA. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -20~-80℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3-6 months, -20~-80℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1. Avoid repeated freezing and thawing.
2. Please operate with lab coats and disposable gloves, for your safety.
3. This product is for research use only.
Product Data
HB220629
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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