Description
Carcinoembryonic antigen (CEA)-related cell adhesion molecule 1 (CEACAM-1; also BGP) is a 160 kDa member of the CEACAM branch of the CEA gene family of the immunoglobulin superfamily . There is no CEACAM-2 in human. The gene for human CEACAM-1 codes for a 526 amino acid (aa) type I transmembrane protein that contains a 34 aa signal sequence, a 394 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 74 aa cytoplasmic region. The ECD contains one N-terminal V-type Ig-like domain, followed by three C2-type Ig-like domains. It shows considerable glycosylation, including high mannose residues and (sialyl) LewisX. The cytoplasmic region shows one ITIM motif and a calmodulin binding site.
Specifications
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Synonyms |
BGP; BGP1; BGPI; CEACAM1; CD66a |
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Uniprot No. |
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Source |
Recombinant Human CEACAM-1/CD66a Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Gln35-Gly428. |
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Molecular Weight |
Approximately 44.4 kDa. Due to glycosylation, the protein migrates to 68-115 kDa based on Tris-Bis PAGE result. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC. |
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Activity |
ELISA Data：Immobilized Human CEACAM-1 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -20~-80℃ for 1 year from date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3-6 months, -20~-80℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Avoid repeated freezing and thawing.
2.Please operate with lab coats and disposable gloves, for your safety.
3.This product is for research use only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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