Description
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR, HER2 , HER3 and HER4 . Within the ECD, human EGFR shares 88% aa sequence identity with mouse and rat EGFR. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis.
Specifications
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Synonyms |
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 |
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Uniprot No. |
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Source |
Recombinant Biotinylated Human EGFR/HER1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu25-Ser645. |
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Source |
The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC |
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Activity |
SPR data: Biotinylated Human EGFR, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.64 nM as determined in SPR assay. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
The products are shipped with ice pack and can be stored at -20℃ for 1 year.
2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 °C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
Note
1.For your safety and health, please wear lab coats and disposable gloves for operation.
2.For research use only!
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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