Description
Siglec-2, also known as B-cell antigen CD22 or B-lymphocyte cell adhesion molecule (BL-CAM), is a B-cell restricted glycoprotein that is expressed in the cytoplasm of progenitor B and pre-B cells and on the surface of mature B cells. The predominant Siglec-2/CD22 beta encodes an 847 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, six Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail with 4 immunoreceptor tyrosine-based inhibition motifs (ITIMs). Siglec-2/CD22 is a coreceptor that physically interacts with B-cell receptor (BCR) and is rapidly phosphorylated upon BCR ligation. It negatively regulates BCR signals by recruiting tyrosine phosphatase SHP-1 to its ITIMs. Phosphorylated Siglec-2/CD22 can also interact with other intracellular effector proteins such as Syk, PLC gamma, PI3 kinase and Grb-2, suggesting it may play a role in positive signaling.
Specifications
|
Synonyms |
CD22 molecule; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814 |
|
Uniprot No. |
P20273-1 |
|
Source |
Recombinant Biotinylated Human Siglec-2/CD22 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Asp20-Arg687. |
|
Source |
The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100μg/mL is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The products are shipped with ice pack and can be stored at -20℃ for 1 year.
2-7 days, 2 to 8 °C under sterile conditions after reconstitution.
3 -6 months, -20 to -80°C under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
Note
1.For your safety and health, please wear lab coats and disposable gloves for operation.
2.For research use only!
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