Description
Specifications
|
Synonyms |
Dkk-1; Dickkopf-1;Dickkopf-related 1; hDkk-1 |
|
Source |
Biotinylated Human DKK1 C terminal Domain Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus. It contains Met178-His266.[Accession | O94907] |
|
Molecular Weight |
The protein has a predicted MW of 38.69 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE |
|
Activity |
ELISA Data: Immobilized Anti-DKK1 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 19.7ng/ml determined by ELISA .Immobilized Human LRP-6, mFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human DKK1 C terminal Domain, hFc Tag with the EC50 of 0.85ug/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 20mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in 20mM NaAc, 150mM NaCl (pH 5.0). |
Storage
The product should be stored at -25~-15℃ for 1 year from date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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