Description
T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Specifications
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Synonyms |
CD3; CD3e; CD3E; CD3d; T3D; CD3D; CD3E&CD3D; CD3 delta&CD3 epsilon |
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Uniprot No. |
P07766(CD3E)&P04234(CD3D) |
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Source |
Recombinant Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Asp23-Glu120 (CD3E)&Phe22-Ala105 (CD3D). |
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Molecular Weight |
The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 18-25 kDa based on Tris-Bis PAGE result. |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC |
|
Activity |
1.ELISA Data：Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA. 2.ELISA Data：Immobilized Human CD3E&CD3D at 2ug/ml (100μl/Well). Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store unopened at -20 to -80°C for 3-6 months. After reconstitution, store at 2-8°C for 2-7 days.
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
3.This product is for research use only.
Product Data
Tris-Bis PAGE
Human CD3E&CD3D on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.
ELISA Data
Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA.
ELISA Data
Immobilized Human CD3E&CD3D at 2ug/ml (100μl/Well).
Dose response curve for Foralumab, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA
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