Description
CD3 is a primary transmembrane protein found on T cells, with four isoforms namely CD3D, CD3E, CD3G and CD3Z, and its CD3D/CD3E, CD3G/CD3E forms heterodimer forms and the α and β strands of TCR form a TCR-CD3 complex. Antigen-presenting cells (APCs)-presented specific MHC peptide complexes can induce T cell activation with TCR-CD3 complexes.
Specifications
|
Synonyms |
CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
|
Uniprot No. |
P07766(CD3E)&P09693(CD3G) |
|
Source |
Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from Expi293 with His tag at the C-terminal. It contains Asp23-Glu120(CD3E)&Gln23-Gln106(CD3G). |
|
Molecular Weight |
The protein has a predicted MW of 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC |
|
Endotoxin |
< 1.0 EU per 1 μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The products are shipped with ice pack and can be stored at -25~-15℃ for 1 year.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal Storage Please avoid freeze-thaw cycles.
Note
1 .For your safety and health, please wear lab coats and disposable gloves for operation.
2. For research use only!
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