Description
Specifications
|
Synonyms |
LAP; GARP;LRRC32; LRRC32&TGF-beta 1 |
|
Source |
Rat GARP&Latent TGF Beta 1 Complex Protein is expressed from HEK293 with His tag and Avi Tag at the C-Terminus. It contains Ile18-Asn628(GARP)&Leu30-Arg278(Latent TGF Beta 1).[Accession | D3ZVD5(GARP)&P17246(Latent TGF Beta)] |
|
Molecular Weight |
The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
ELISA Data: Immobilized Rat GARP&Latent TGF Beta 1 Complex, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 2.0μg/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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