Description
covalent heterodimer with CD79A. This complex interacts non-covalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79AB initiates signaling. Mature human CD79B is a 201 amino acid (aa) type I transmembrane glycoprotein (aa 29229). It contains an extracellular region with one V-type Ig-like domain (aa 38-138) and an ITAM-containing cytoplasmic domain (aa 185-213). There is an alternative splice form that shows a deletion of aa 41-144 and appears after B cell activation. Human CD79A and B share only 26% aa identity. Over aa 29-159, human CD79B shares 54% aa identity with mouse CD79B.
Specifications
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Synonyms |
B29; CD79b molecule; CD79B; IGB; IGBAGM6; Ig-beta; Ig-β |
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Uniprot No. |
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Source |
Recombinant Human CD79B Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Ala29-Asp159. |
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Molecular Weight |
The protein has a predicted MW of 18.1 kDa. Due to glycosylation, the protein migrates to 33-42 kDa based on Tris-Bis PAGE result. |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC |
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Activity |
ELISA Data：Immobilized Human CD79B, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-CD79b Ab., hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. Batch to Batch Consistency: Immobilized Human CD79B, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-CD79B Ab., hFc Tag with the EC50 of 15.9/15.3/15.2ng/ml determined by ELISA. |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
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