Description
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to ESelectin ligands on leukocytes. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.
Specifications
|
Synonyms |
CD62E; ELAM; ELAM1; ESEL; E-Selectin; LECAM2; SELE; selectin E; RP1-117P20.2 |
|
Uniprot No. |
P16581 |
|
Source |
Recombinant Human E-Selectin/CD62E Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Trp22-Pro556. |
|
Molecular Weight |
Approximately 61.6 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
ELISA Data：Immobilized Human E-Selectin at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA. |
|
Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transported with ice packs. Store at -20℃ to -80℃, valid for one year.
After reconstitution, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2 to 8°C for 2 to 7 days.。
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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