Description
Specifications
|
Synonyms |
GDF-15; MIC-1; NAG-1; PDF; PLAB; PTGFB; GDF15; MIC1; RG-1; Placental TGF-beta; PTGF-beta; PTGFBPTGF-beta; |
|
Species |
Human |
|
Source |
E.coli |
|
Sequence |
Ala197-Ile308 |
|
Accession |
Q99988 |
|
Tag |
N-His |
|
Molecular Weight |
The protein has a predicted MW of 13.5 kDa. The protein migrates to 15-16 kDa based on Bis-Tris PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by Bis-Tris PAGE |
|
Activity |
ELISA Data: Immobilized Human GDF15, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Human GFRAL, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA.
Blocking Data: Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.
SPR Data: Human GFRAL, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF15, His Tag with an affinity constant of 0.014 nM as determined in SPR assay . |
|
Formulation |
Lyophilized from 0.22μm filtered solution in 50mM HAc (pH 2.9). Normally 8% trehalose is added as protectant before lyophilization. |
|
Appearance |
Lyophilized powder |
Storage
Store at -25~-15℃, valid for 1 year after receipt. After reconstitution, store at -85~-65℃, valid for 3 months.
Reconstitution method
Centrifuge before opening the lid. Recommended reconstitution concentration is above 100 μg/ml. Dissolve the lyophilized protein in 50 mM HAC (pH 2.9).
Note
1. For your safety and health, please wear a lab coat and disposable gloves when operating.
2. This product is for scientific research purposes only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
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