Описание
IL-27 (interleukin 27) is a cytokine belonging to the IL-12 family, a heterodimeric cytokine consisting of EBI3 and p28 subunits.IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines.IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and pro-inflammatory cytokines.IL-27 is produced through the induction of the expression of the transcription factor T-bet and up-regulation of IL-12Rβ 2 to promote the differentiation of CD4+ T cells into the Th1 lineage, which in turn inhibits the differentiation and proliferation of Th2 and Th17.IL-27 is a member of the recently discovered family of proinflammatory and immunomodulatory cytokines IL-6/IL-12.IL-27 acts by binding to its receptors WSX-1 and gp130, which leads to the activation of the Jak/STAT signaling cascade, suggesting that IL-27 is involved in the regulation of immune processes.
Recombinant Human IL-27 produced by HEK293 cells is a non-disulfide-linked heterodimeric protein consisting of the 209 amino acid-long EBI3 subunit and the 215 amino acid-long IL-27-p28 subunit, with a total sequence length of 424 amino acid residues. This product is available as Fluids, high purity, high activity, low endotoxin, NO labeling.
Product Properties
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Synonyms |
Human IL-27, h-IL-27, rh-IL-27, recombinant IL-27, interleukin-27 |
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Species |
Human |
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Source |
HEK293 Ceslls |
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Sequence |
IL-27 EBI-3(Arg21-Lys229),IL-27 p28(Phe29-Pro243) |
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Accession |
Q14213.2 (IL-27EBI3) & AAM34498 (IL-27p28 |
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Tag |
NO |
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Predicted molecularmass |
56.0 kDa |
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Endotoxin |
<0.010 EU per 1 μg of the protein by the LAL method |
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Purity |
> 95%, determined by SDS-PAGE |
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Activity |
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The EC50 for this effect is 1-6 ng/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Appearance |
Fluids |
Shipping and Storage
-85~-65℃ storage, valid for 1 year after receipt.
Avoid repeated freezing and thawing, centrifuge before opening the vial. It is recommended to aliquot for one experimental use, after aliquoting, freeze at -80℃.
Cautions
1. For your safety and health, please wear lab coat and disposable gloves.
2. This product is for scientific research purposes only.
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Расследование
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