Описание
PDGF R alpha (platelet-derived growth factor receptor alpha) is a type I transmembrane glycoprotein in the class III subfamily of receptor tyrosine kinases (RTK). Within the ECD, human PDGF R alpha shares 85%, 83%, 95%, 93%, and 88% aa sequence identity with mouse, rat, equine, canine and bovine PDGF R alpha respectively. PDGF R alpha autophosphorylates upon dimerization, activating signaling cascades in PI 3-kinase Ras-MAP kinase, and PLC-gamma pathways. Signaling is downregulated by SHP-2 phosphatase activity and by receptor endocytosis and lysosomal degradation. PDGF R alpha is expressed at low levels in most mesenchymal cells, but is strongly expressed in oligodendrocyte, lung, skin and intestinal progenitor cells and induced by inflammation or growth in culture.
Specifications
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Synonyms |
PDGFRA, CD140A, PDGFR2, RHEPDGFRA, MGC74795, PDGFR-2 |
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Uniprot No. |
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Source |
Recombinant Human PDGF R alpha / PDGFRA Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gln24-Glu524. |
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Molecular Weight |
Approximately 59.2 kDa. Due to glycosylation, the protein migrates to 70-83 kDa based on Tris-Bis PAGE result. |
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Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
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Purity |
> 95% as determined by SDS-PAGE and HPLC |
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Activity |
Testing in progress |
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Endotoxin |
< 1.0 EU per 1μg of the protein by the LAL method. |
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Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
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Reconstitution |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL` is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Storage
Transport with ice packs. Store at -20℃ to -80℃, valid for one year.
Once reconstituted, store unopened at -20 to -80°C for 3 to 6 months. After reconstitution, store at 2-8°C for 2-7 days.
It is recommended to store in aliquots and freeze for the first use to avoid repeated freezing and thawing.
Note
1. Avoid repeated freezing and thawing.
2. For your safety and health, please wear a lab coat and disposable gloves when operating.
3. This product is for scientific research purposes only.
Product Data
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Расследование
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