描述
Interleukin-5 (IL-5) is a hematopoietic growth factor that stimulates the proliferation and activation of eosinophils.IL-5 is produced by mast cells, T-cells, and eosinophils, and plays an important role in inducing cell-mediated immunity against parasitic infections and certain tumors.Elevated levels of IL-5 can lead to eosinophilia, which may cause asthma and other allergic diseases. Human and mouse IL-5 are cross-species reactive.
Recombinant Human IL-5 is a 26.5 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains. This product, Recombinant Human IL-5, is supplied as a lyophilized powder with high activity, high purity and low endotoxin.
Specification
|
Synonyms |
Interleukin-5; B-cell differentiation factor I, Eosinophil differentiation factor, TRF |
|
Source |
E.coli-derived human IL-5 protein, Ile20-Ser134,with an N-terminal Met. |
|
Accession |
P05113 |
|
Molecular Weight |
Approximately 26.5 kDa. |
|
AA Sequence |
MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES |
|
Endotoxin |
< 1.0 EU/μg by the LAL method. |
|
Purity |
> 98% by SDS-PAGE and HPLC. |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.5, 3% trehalose, 0.1% Tween-80. |
|
Physical Appearance |
White lyophilized powder |
Storage
-25 ~ -15 ° C storage, valid for 1 year after receipt.
After re-solution, store at 2 ~8 °C, 7 days expiration date. After re-dissolution, store at -85~-65°C, 3 months expiration date.
Compounding Methods
Centrifuge before opening the cap to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier protein (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing.
Cautions
1. Please operate with lab coats and disposable gloves,for your safety.
2. This product is for research use only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.