描述
分泌型马 IL-1 受体拮抗剂 (IL-1ra) 是一种大概 22-25 kDa 的糖蛋白，由多种细胞类型产生，可拮抗 IL-1 活性。它是 IL-1 蛋白家族的成员，包括 IL-1 alpha 和 IL-1 beta。尽管氨基酸 (aa) 身份不明 (< 30%) among the three IL-1 family members, all molecules bind to the same receptors, all show a beta -trefoil structure, and all are believed to have evolved from a common ancestral gene. Equine IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence plus a 152 aa mature region. There is one intrachain disulfide bond and one potential N-linked glycosylation site. Mature equine sIL-1ra is 78%, 78%, 80%, 82%, and 76% aa identical to mature mouse, human, porcine, canine and bovine IL-1ra, respectively. In human, three non-secreted IL-1ra isoforms have also been identified. It is unknown if such an analogous situation exists in equine. Cells known to secrete IL-1ra include fibroblasts, vascular smooth muscle cells, intestinal columnar epithelium, chondrocytes, macrophages, mast cells, neutrophils and hepatocytes. There are two type I transmembrane glycoprotein receptors for IL-1ra. The first is the bioactive 80 kDa type I IL-1 receptor (IL-1 RI), and the second is the inert (decoy) 65 kDa type II IL-1 receptor. IL-1ra binding to IL-1 RI competitively blocks IL-1 ( alpha or beta ) binding to the same receptor. This results in receptor ligation without activation. The type II IL-1 receptor is inert, and any binding of IL-1ra not only fails to block co-existing IL-1 activity, but may actually potentiate it by removing an IL-1 antagonist. Functionally, all activities attributed to IL-1ra are.
产品 特性
| 同义词 | IL-1RN，IRAP |
| 加入 | O18999 |
| 基因识别 | 100034236 |
| 来源 | 大肠杆菌来源 马 IL-1RA，His26-Gln177。 |
| 分子量 | 大约 17.4 kDa。 |
| AA 序列 | HPLGKRPCKM QAFRIWDVNQ KTFYMRNNQL VAGYLQESNT KLQEKIDVVP IEPDALFLGL HGRKLCLACV KSGDEIRFQL EAVNITDLSK NKEENKRFTF IRNSGPTTS FESAACPGWF LCTAQEADRP VSLTNKPKES FMVTKFYLQE DQ |
| 标签 | 没有任何 |
| 外貌 | 无菌过滤白色冻干（冷冻干燥）粉末。 |
| 纯度 | 通过 SDS-PAGE 和 HPLC 分析，结果显示 > 95%。 |
| 生物活性 | ED50 通过抑制小鼠 D10S 细胞的 IL-1α 依赖性增殖来确定浓度小于 3.0 μg/mL，相当于在 50 pg/mL 存在下比活性 > 333 IU/mg 重组人白细胞介素-1α。 与标准相比，具有完全的生物活性。 |
| 内毒素 | < 1.0 欧盟 每 1μg 这 蛋白质 这 LAL 方法。 |
| 配方 | 从 PBS 中 0.2 µm 过滤的浓缩溶液中冻干，pH 值为 7.4。 |
| 重建 | 我们建议在打开前短暂离心此小瓶，使内容物沉至底部。在无菌蒸馏水或含有 0.1% BSA 的水性缓冲液中重新配制，浓度为 0.1-1.0 mg/mL。应将原液分装成工作等分试样，并储存在 ≤ -20°C 的温度下。应在适当的缓冲溶液中进行进一步稀释。 |
运输和储存
产品附带冰袋运输，可储存在-20℃至-80℃ 为了 1 年。
建议首次使用时将蛋白质分装成较小的量 并避免反复冻融。
注意事项
1. 避免反复冻融。
2. 为了您的安全和健康，操作时请穿着工作服和戴一次性手套。
3. 仅供研究使用！
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