描述
|
Cat# |
Size |
|
|
Recombinant Human IGF-1 Protein
|
92211ES10 |
10 μg |
|
92211ES60 |
100 μg |
|
|
92211ES76 |
500 μg |
|
|
92211ES80 |
1 mg |
IGFs are mitogenic peptide growth factors that stimulate the proliferation and survival of a variety of cell types in vitro, including muscle, bone, and cartilage tissues.IGFs are produced primarily by the liver, although a variety of tissues produce IGFs at different times.IGFs belong to the insulin gene family, which also contains insulin and relaxin.IGFs are structurally and functionally similar to insulin but have a much much higher growth-promoting activity.The expression of IGF-II is influenced by placental prolactin, whereas the expression of IGF-I is regulated by growth hormone.Both IGF-I and IGF-II signal through the tyrosine kinase type I receptor (IGF-IR), but IGF-II can also signal through the IGF-II/mannose-6-phosphate receptor. Mature IGFs are produced by proteolytic processing of inactive precursor proteins that contain N-terminal and C-terminal prepeptide regions.
This product, Recombinant Human IGF-1, is supplied as a lyophilized powder with high activity, high purity and low endotoxin.
Specification
|
Synonyms |
Somatomedin C, IGF-I, IGF-IA, Mechano growth factor, MGF |
|
Source |
E.coli-derived human IL-IGF-1 protein, Gly49-Ala118 without tag. |
|
Accession |
P05019 |
|
Molecular Weight |
Approximately 7.6 kDa. |
|
AA Sequence |
GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA |
|
Endotoxin |
< 0.01 EU/μg by the LAL method. |
|
Purity |
> 97%, SDS-PAGE and HPLC detection |
|
Biological Activity |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
|
Formulation |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.0. |
|
Physical Appearance |
White lyophilized powder |
Storage
-25 ~ -15 ° C storage, valid for 1 year after receipt.
After re-solution, store at 2 ~8 °C, 30 days expiration date. After re-dissolving, store at -85~-65°C, 3 months expiration date.
Compounding Methods
Centrifuge before uncapping to bring contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL, which can be diluted even further according to subsequent experiments; for long-term storage, it is recommended that carrier proteins (0.1% BSA) be added to the dilution buffer; dispense for a single experimental dose and freeze at -80°C to avoid repeated freezing and thawing.
Cautions
1.For your safety and health, please wear lab coat and disposable gloves.
2.This product is for scientific research purposes only.
付款和安全
您的付款信息已安全处理。我们无法存储信用卡详细信息，也无法访问您的信用卡信息。
询问
你也可能喜欢
常问问题
The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.