描述
Specifications
|
Synonyms |
p75TBPII; Etanercept;CD120b; TNF RII |
|
Source |
Human TNF R2/CD120b/TNFRSF1B Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Leu23-Asp257.[Accession | P20333] |
|
Molecular Weight |
The protein has a predicted MW of 51.5 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on SDS-PAGE result. |
|
Endotoxin |
Less than 1EU per μg by the LAL method. |
|
Purity |
> 95% as determined by SDS-PAGE and HPLC. |
|
Activity |
ELISA Data: Immobilized Human TNFR2, mFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-TNFR2 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA .Immobilized Human TNF alpha, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TNFR2, mFc Tag with the EC50 of 61.0ng/ml determined by ELISA. |
|
Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~ 8 °C under sterile conditions after reconstitution.
3 -6 months, -85~-65℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
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The product is for research purposes only and is not intended for therapeutic or diagnostic use in humans or animals. Products and content are protected by patents, trademarks, and copyrights owned by Yeasen Biotechnology. Trademark symbols indicate the country of origin, not necessarily registration in all regions.
Certain applications may require additional third-party intellectual property rights.
Yeasen is dedicated to ethical science, believing our research should address critical questions while ensuring safety and ethical standards.