Synonyms

ARP2; ANGPTL2; angiopoietin-like 2; MGC8889

Source

Biotinylated Human ANGPTL2/Angiopoietin-like 2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Thr260-His493.[Accession | Q9UKU9-1]

Molecular Weight

The protein has a predicted MW of 30.1 kDa. Due to glycosylation, the protein migrates to 31-35 kDa based on Tris-Bis PAGE result.

Endotoxin

Less than 1EU per μg by the LAL method.

Purity

> 95% as determined by SDS-PAGE and HPLC.

Activity

SPR Data: Human LILRB2, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human ANGPTL2, His Tag with an affinity constant of 65.63 nM as determined in SPR assay .

Formulation

Lyophilized from 0.22μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.