وصف
High-mobility Group Box 1 protein (HMGB1), is a member of the high mobility group box family of non-histone chromosomal proteins. Posttranslational modification of HMGB1, including acetylation, phosphorylation, and methylation, affects HMGB1 localization, receptor interactions, and bioactivity. HMGB1 can be localized to the nucleus or cytoplasm and can also be secreted despite its lack of a signal peptide. HMGB1 can be secreted by multiple cell types, and it is also released upon cell necrosis, apoptosis, and pyroptosis.
Specifications
|
Synonyms |
HMGB1, HMG1, HMG3, SBP-1, HMG-1 |
|
Uniprot No. |
P09429-1 |
|
Source |
Recombinant Human HMGB1 Protein is expressed from HEK293 Cells with His tag at the C-terminal. It contains Met1-Glu215. |
|
Molecular Weight |
Approximately 26 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. |
|
Purity |
> 95% as determined by SDS-PAGE. |
|
Endotoxin |
<1.0 EU per 1μg of the protein by the LAL method. |
|
Formulation |
Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
|
Reconstitution |
Centrifuge tubes before opening. Dissolve lyophilized protein with sterile water to ensure the concentration is greater than 100 ug/mL. |
Storage
The product should be stored at -25~-15℃ for 1 year from the date of receipt.
2-7 days, 2 ~8 °C under sterile conditions after reconstitution.
3 months, -25~-15℃ under sterile conditions after reconstitution.
Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.
Note
1.Please operate with lab coats and disposable gloves for your safety.
2.This product is for research use only.
Product Data
Figure 1. Human HMGB1 on SDS-PAGE under reduced condition. The purity is greater than 95%.
Figure 2. The purity of Human HMGB1 is greater than 95% as determined by SEC-HPLC.
الدفع والأمن
تتم معالجة معلومات الدفع الخاصة بك بشكل آمن. لا نقوم بتخزين تفاصيل بطاقة الائتمان ولا يمكننا الوصول إلى معلومات بطاقة الائتمان الخاصة بك.
سؤال
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التعليمات
المنتج مخصص لأغراض البحث فقط وليس مخصصًا للاستخدام العلاجي أو التشخيصي لدى البشر أو الحيوانات. المنتجات والمحتوى محميان بموجب براءات الاختراع والعلامات التجارية وحقوق الطبع والنشر المملوكة لشركة Yeasen Biotechnology. تشير رموز العلامة التجارية إلى بلد المنشأ، وليس بالضرورة التسجيل في جميع المناطق.
قد تتطلب بعض التطبيقات حقوق الملكية الفكرية الإضافية لجهات خارجية.
تلتزم شركة Yeasen بالعلوم الأخلاقية، حيث تؤمن بأن أبحاثنا يجب أن تعالج الأسئلة الحرجة مع ضمان معايير السلامة والأخلاق.