Due to the complexity, error-proneness, and inconvenience of preparing conventional qPCR reagent reaction systems,fully pre-mixed reagents (which support pre-mixing and storage of primers and probes) have been widely used in various fields of molecular biology research, such as infectious disease transmission, reproductive genetics, early tumor screening and companion diagnostics, forensic identification, animal disease testing, food safety monitoring, and evaluation of biological products. 

To meet the needs of customers for more detection scenarios and efficient multiplex amplification, YEASEN has launched a new 3rd generation universal fully pre-mixed qPCR master mix. This product has significantly improved the versatility of the reagents for multiplex amplification in multiple scenarios (fully verified in pathogen, human, and plant multi-target amplification), addressing the pain points of stability in multiplex detection and target versatility. It also enhances key performance indicators such as sensitivity and specificity, thereby improving detection efficiency. 

Product Advantages

• Excellent reagent versatility – Compatible with a variety of multiplex primer-probe sets (validated for over 40 different types of targets), with excellent amplification performance.
• Ultra-strong pre-mixed stability – Can be stored at 37°C for up to 14 days, at 4°C for up to 28 days, and maintains stable performance after 50 freeze-thaw cycles.
• High sensitivity and specificity – The sensitivity is capable of detecting 0.25 copies per microliter, with specificity ensuring no peaks in 48 negative wells.
• Supports rapid protocols – Compatible with rapid protocols, results can be obtained in just 30 minutes.
• dUTP/UDG anti-contamination system – Incorporates a dUTP/UDG anti-contamination system to effectively prevent aerosol contamination.
• Compatibility with multiple platforms and systems – Compatible with platforms such as Bio-Rad CFX96, ABI Q5, 7500, Slan, Tianlong, and others.

Performance Highlights

1、Excellent reagent versatility

The brand-new 3rd generation universal fully pre-mixed reagent has improved sensitivity and versatility for multiple targets across various scenarios, building upon the previous generation of fully pre-mixed reagents. It has undergone extensive multiplex validation with a variety of targets from pathogens, human sources, and plants.