Tag Antibodies Introduction
Tag antibodies are used for the detection and purification of tag sequences on various commercial expression vectors to analyze and determine the expression levels and functions of the target proteins. The principle behind their use is the antigen-antibody reaction, where these tag antibodies can specifically bind to the corresponding tag-fused proteins. Here is a brief list of some common protein tags suitable for use with tag antibodies:
Table 1. Introduction to Different Protein Tags
|
Tag |
Sequence |
Features |
Applicable Scenarios |
|
Flag |
DYKDDDDK |
1. Small molecular weight, does not change the function of the fusion protein, more hydrophilic than similar tags; 2. Contains enterokinase recognition site (DDDDK), which is convenient for tag removal when fused at the N-terminus of the target protein. |
Suitable for purification and detection of low-expression level fusion proteins in eukaryotic expression systems. |
|
HA |
YPYDVPDYA |
Derived from the hemagglutinin surface antigen determinant of the influenza virus; avoid using in experiments related to apoptotic cells. |
Suitable for protein purification and detection. |
|
Myc |
Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu |
Derived from the human proto-oncogene Myc; avoid using in experiments related to human cells. |
Generally not used for protein purification, suitable for detection. |
Yeasen tag antibody affinity purification products are made from high-quality mouse IgG monoclonal antibodies as raw materials. Through a process called "covalent coupling," these antibodies are directionally and efficiently coupled to agarose matrices or the surfaces of nano-magnetic beads. This not only reduces non-specific protein binding but also achieves a higher antibody load on the medium. Additionally, it provides tag antibody purification products suitable for various purification requirements, including different tag antibody purification resins and immunomagnetic beads, facilitating the purification of tagged proteins and immunoprecipitation experiments using manual or automated methods.
Yeasen Tag Antibody Purification Product Selection Guide
|
Product Series |
Purification Resin Series |
ImmunoMagnetic Bead Series |
|||||
|
Product Number |
20584ES |
20586ES |
20587ES |
20565ES |
20566ES |
20567ES |
20564ES |
|
Product Name |
Anti-DYKDDDDK(Flag) Affinity Gel |
Anti-HA Affinity Gel |
Anti-Myc Affinity Gel |
Anti-DYKDDDDK(Flag) MagBeads |
Anti-HA MagBeads |
Anti-MycMagBeads |
Anti-GFP MagBeads |
|
Ligands |
High-quality mouse IgG monoclonal antibodies |
High-quality mouse anti-GFP/Flag/HA/Myc monoclonal antibodies |
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|
Protein Binding Capacity |
≥1.1 mg Flag-tagged fusion protein/mL gel ≥1.5 mg HA-tagged fusion protein/mL gel ≥0.9 mg Myc-tagged fusion protein/mL gel |
≥0.8 mg GFP-tagged fusion protein/mg beads ≥0.6 mg Flag-tagged fusion protein/mg beads ≥0.4 mg HA-tagged fusion protein/mg beads ≥0.5 mg Myc-tagged fusion protein/mg beads
|
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|
Matrix |
4% cross-linked agarose gel |
Silica-based magnetic beads |
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|
Particle Size |
45-165 µm |
200 nm |
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|
Advantages |
Low non-specific binding, high capacity |
High purity, high capacity, high specificity, convenient use with magnetic rack |
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|
Recommended Applications |
Affinity purification of tagged fusion proteins at any scale, immunoprecipitation (IP) |
Immunoprecipitation (IP), Co-immunoprecipitation (COIP) |
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Test Data
1.Yeasen Anti-Flag Affinity Gel (20584ES) vs. Brand S* Purification Comparison Test.
Figure 1. Yeasen (a) vs. Brand S* (b) Anti-Flag Gel Comparison Test Gel Image
M: Protein Marker; 1: Pre-column lysate of Flag-N, M, C proteins; 2: Post-column permeate of Flag-N, M, C proteins; 3: Elution (pH 2.7 elution); 4: Elution (pH 12 elution)
2.The arrows in the figure below indicate the target tagged proteins, and the three bands from top to bottom after boiling the magnetic beads directly are BSA, heavy chain of antibody, and light chain of antibody.
Figure 2. Anti-Flag MagBeads Purification of Flag-Tagged Proteins
1: E. coli lysate containing Flag-tagged protein 10 μL; 2: Supernatant after incubation of E. coli lysate containing Flag-tagged protein with magnetic beads 10 μL; 3: Direct boiling of Flag-tagged protein with Anti-Flag MagBeads 10 μL; 4: Direct boiling of Anti-Flag MagBeads; 5: Marker
3.
Figure 3. Anti-GFP MagBeads Purification of GFP-Tagged Proteins
1: E. coli lysate containing GFP-tagged protein 10 μL; 2: Supernatant after incubation of E. coli lysate containing GFP-tagged protein with magnetic beads 10 μL; 3: Direct boiling of GFP-tagged protein with Anti-GFP MagBeads 10 μL; 4: Direct boiling of Anti-GFP MagBeads; 5: Marker
Product Information
|
Product Name |
Product Number |
Specification |
|
Anti-DYKDDDDK(Flag) Affinity Gel |
20584ES03/08/25 |
1 mL/5 mL/25 mL |
|
Anti-HA Affinity Gel |
20586ES03/08/25 |
1 mL/5 mL/25 mL |
|
Anti-Myc Affinity Gel |
20587ES03/08/25 |
1 mL/5 mL/25 mL |
|
Anti-DYKDDDDK(Flag) MagBeads |
20565ES03/08 |
1 mL/5 mL |
|
Anti-HA MagBeads |
20566ES03/08 |
1 mL/5 mL |
|
Anti-Myc MagBeads |
20567ES03/08 |
1 mL/5 mL |
|
Anti-GFP MagBeads |
20564ES03/08 |
1 mL/5 mL |